SmartPeptide

Storage & reconstitution reference

Lyophilized (freeze-dried) peptides arrive as a powder and must be mixed with a sterile diluent before use. This page covers the standard handling practices found in compounding-pharmacy reference guides: storage temperatures, the mixing process, and the microgram-to- milligram conversion table.

Handling reference — not medical advice

SmartPeptide does not prescribe, diagnose, or treat, and does not tell you whether to use any peptide. This page documents standard laboratory and pharmacy handling practice so you can understand what proper storage and preparation involve. Many research peptides are not FDA-approved for human use. Sterile technique matters — contamination and dosing errors carry real risk. Work with a licensed clinician.

Before reconstitution

Storing the lyophilized powder.

Room temperatureUp to ~1 month

Generally stable, but not the preferred long-term option.

Refrigerated (4°C / 39°F)Up to ~1 year

The standard choice for medium-term storage.

Frozen (−20°C / −4°F)Longer than 1 year

Maximum stability for extended storage.

Light & moistureProtect from both

Store in a dark, dry place in airtight vials. UV and humidity degrade peptides.

After reconstitution

Once the powder is mixed into solution.

Refrigerate at 4°CRequired

Reconstituted peptides must stay cold to maintain potency.

Do not freezeAvoid

Freezing after reconstitution causes degradation.

Inspect the solutionEvery use

Some peptides (AOD-9604, TB-500) can normally look cloudy or viscous. Discard on unexpected particulates, discoloration, or odor.

Label the vialAlways

Write the reconstitution date on every vial.

The reconstitution process

Standard pharmacy handling practice
1
Use bacteriostatic (BAC) water

Bacteriostatic water contains 0.9% benzyl alcohol, which suppresses bacterial growth and supports peptide stability across multiple draws. Sterile water without a preservative is single-use only.

2
Inject air into the vial first

Push roughly 1 mL of air into the vial before adding diluent. This equalizes the negative pressure inside the lyophilized vial, making the liquid easier to withdraw later.

3
Add the diluent slowly, down the side

Aim the stream at the inside wall of the vial rather than directly onto the powder. This minimizes foaming and mechanical stress. Peptides are fragile — vigorous mixing damages their structure.

4
Let it dissolve on its own

Do not shake. Allow the powder to go into solution naturally; gently roll or invert the vial if needed. Some peptides dissolve within seconds, others take several minutes.

5
Label and refrigerate

Write the reconstitution date on the vial and store at 4°C. Inspect for clarity before each draw.

Microgram ↔ milligram conversion

1,000 µg = 1 mg

Peptide doses are quoted in both units, which is a common source of 100× and 1,000× dosing errors. µg (microgram, sometimes written “mcg”) is one thousandth of a mg (milligram).

100µg0.1mg
200µg0.2mg
250µg0.25mg
300µg0.3mg
400µg0.4mg
500µg0.5mg
600µg0.6mg
700µg0.7mg
800µg0.8mg
900µg0.9mg
1,000µg1mg
1,200µg1.2mg
1,500µg1.5mg
2,000µg2mg
2,500µg2.5mg
3,000µg3mg
3,500µg3.5mg
4,000µg4mg
5,000µg5mg

Doses-per-vial stability guardrail

Compounding-pharmacy references commonly advise against reconstituting at a concentration that yields more than roughly six doses per vial for a single individual. The reasoning: each needle entry through the stopper is an opportunity for contamination, and the bacteriostatic preservative loses effectiveness over an extended draw window. Vials holding many small doses are typically a clinical-setting pattern, not a home-use one.

Run the numbers

Enter a vial size, water volume, and target dose to get mL to draw and insulin-syringe units. Pure arithmetic, no recommendations.

Open the calculator

Educational only — not medical advice. SmartPeptide does not prescribe, diagnose, or treat. Always consult a licensed healthcare provider before using any peptide, supplement, medication, or protocol.